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Tobramycin works by binding to a site on the bacterial 30S and 50S ribosome , preventing formation of the 70S complex. As a result, mRNA cannot be translated into protein, and cell death ensues. According to Kotra, Haddad and collaborators "aminoglycosides, including Tobramycin, are multifunctional hydrophilic sugars that possess several amino and hydroxy functionalities. The amine moieties are mostly protonated in biological media; hence, these antibiotics can be considered polycationic species for the purpose of understanding their biological interactions. Since they are polycationic, they show a binding affinity for nucleic acids. Specifically, aminoglycosides possess high affinities for certain portions of RNAs, especially the prokaryotic rRNA. In addition, aminoglycosides bind to the hammerhead ribozyme, tRNA(Phe), the Rev response element (RRE) transcriptional activation region in human immunodeficiency virus (HIV), the ribozyme from hepatitis delta virus, and group I self-splicing introns.[4]"

Hi Mike,
Although I’ve been following your work for months, this is the first time I comment to ask you a question about your progressive overload method.
I’ve been stuck with the same weight in side lateral dumbbell raises for a while. I can perform the exercise with perfect form with 10kg (20lb) dumbbells for 10 or more reps. However, when I go up to 12,5kg (25lb) dumbbells I cannot get any rep with good form. Using momentum I am able to go to 7-8 reps at 25lb, but I can’t get any of them with good form.

My horror story startd 6 yrs ago,was sent 4 physio,diag as tendonitis,did physio 4 5wks couldnt do had scan 4 hernia,not that had x rays,couldnt find this time when i sat down,it took 2 mins b4 i cud walk next went 2 c 2 visits he said u will need new hip  was seen by surgeon 2 av inj into hip to define god that months later had new from day 1. So much later had aspiration test and painkilling didnt work seeing consultant inbetween. Then had ultrasound and inj into did nothing,But rad/ographer said something is going again nothing surgeon got another surgeon 4 second said it was a trapped now i av gone nearly 2 yrs on crutches,With pain in my groin/ to the touch over the scar,And it feels like i am sitting on a piece of wood,As well as being diagnosed as having brain is the problem is i dont look 63 and i look wk went 4 private consultation. Consultant doesnt want to i am well up the 2 av droned on. TEDDY

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