I manage the diverse algae research projects performed by PhD and honours students working with Schenk Lab. My main interests centre around the production and processing of microalgae for nutraceuticals such as omega 3 fatty acids, carotenoids and pigments.
I have extensive experience in large scale low cost algae cultivation, the main focus being production of biomass as a high protein feed supplement for livestock and aquaculture. The long term vision for microalgae is the sustainable production of biofuels, this is what sparked my initial interest in microalgae.
CHO-IR cells expressing human insulin receptor are grown to 80% confluence in Hamm’s F12 medium with 10% fetal bovine serum and without hypoxanthine. Trypsinized cells are seeded in 6-well plates at 1 × 106 cells/well in 2 ml of medium without fetal bovine serum. After 24 h, medium is replaced with 1 ml of serum-free medium containing GSK-3 inhibitor or control (final DMSO concentration <%) for 30 min at 37°C. Cells are lysed and centrifuged 15 min at 4°C/14000g. The activity ratio of GS is calculated as the GS activity in the absence of glucose-6-phosphate divided by the activity in the presence of 5 mmol/l glucose-6-phosphate, using the filter paper assay of Thomas et al.