Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
While yucca is definitely a safer alternative to synthetic steroids, it should be used in moderation. It should be used to treat a specific condition rather than for everyday, regular use. If it needs to be used over a longer timeframe, make sure to give your dog a couple of days off every week from yucca. Too much yucca can irritate the digestive tract and may reverse its benefits, according to Herbs for Pets. ( Dosing for the dried and powdered root is a 1/2 teaspoon per pound of food. But check with your holistic vet if you are ever unsure.)
1. Discontinue steroids—In the acute form of IOP elevation from steroids, discontinuing steroids can cause the IOP to normalize in days. In the chronic form, elevation of IOP can last one to four weeks. 26 In a small subset of patients, the IOP may remain chronically elevated despite discontinuation of steroids. In one series, % of eyes converted to glaucoma. Interestingly, all of these patients had a family history of The duration of steroid treatment seems to play a role as well. In one series, the IOP remained elevated in patients where the steroid was used for more than four years. 28 2. Removal of depot steroids—One can cause a decrease in IOP by excising depot ,29,30 For intravitreal steroids, vitrectomy can also be used to reduce 3. Glaucoma treatment—Treatment of steroid glaucoma includes the use of topical glaucoma medications, laser trabeculoplasty, filtering surgery, glaucoma drainage implant surgery, or one of the other means of treatment of primary open angle glaucoma.